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Background: The streptococcus mutans and lactobacilli have been considered to be the most common causative bacteria for dental decay. Antibacterial action of polycarboxylate cement reinforced by different amount of fluoroapatite and calcium fluoride is important property for the filling restoration to prevent secondary caries and to evaluate these materials to be used as base filling in which the antibacterial activity is important property for successful filling and cementing material for crowns and bridges ,thus the aim of the study was the analysis the antimicrobial activity of polycarboxylate cement reinforced by different amount of fluoroapatite and calcium fluoride. Materials& methods: fluoroapatite materials and calcium fluoride were added to polycarboxylate cement at different ratios; 0% (Control), 5% ,10%,15%, 20%, 25% (by weight). Agar diffusion method will used to evaluate the antibacterial effect polycarboxylate cement and polycarboxylate cement reinforced by different amounts of fluoroapatite and calicium fluoirde (5% ,10%,15%, 20% and 25%) in this study two bacterial strains are used ; streptococcus mutans and lactobacilli which are cultured. A six holes (5mm depth and 4mm diameter) in each Petri dish( 20 petri dish for each of two micro organism of this study) ; 10 petri dish for polycarboxylate cement and polycarboxylate cement reinforced by different amounts of fluoroapatite (5% ,10%,15%, 20% and 25%) and 10 petri dish for polycarboxylate cement and polycarboxylate cement reinforced by different amounts of calicium fluoirde (5% ,10%,15%, 20% and 25% ) . in this study the material were added in these Wells (one Well for each ratio was used) and after incubation of these Petri dishes , the inhibition zones of each microorganism of this study were measured in millimeter around each of these Wells. Results: the conclusion n this study showed that the polycarboxylate cement and polycarboxylate cement reinforced by different amounts of fluoroapatite and calicium fluoride have strong antibacterial activity against streptococus mutans and lactobacilli Bacteria. Also the results showed that the increase the ratio of fluoroapatite will statistically increase their antibacterial activity aganist streptococcus mutans and lactobacilli as far as 20%.