31Jul 2017

EFFECT OF PHOSPHINE ON LEVELS OF ALKALINE AND ACID PHOSPHATASES IN SUSCEPTIBLE AND RESISTANT STRAINS OF TROGODERMA GRANARIUM (EVERTS) (COLEOPTERA: DERMESTIDAE).

In the present study change in level of acid and alkaline Phosphatases in phosphine resistant and susceptible strains of T. granarium at LC10, LC20 andLC30 was studied. Activity of acid phosphatase in all resistant and susceptible beetles was significantly increased at LC10 and LC20 as compared to control beetles, while alkaline phosphatase decreased at LC10 and had non-significant difference at LC20 in all resistant and susceptible beetles. At LC30 both Phosphatases significantly increased in Khaniwal population. Hyderabad population also showed significant elevated level at C30. Drastic decrease observed in Rawalpindi strain. The results of present study suggested that both acid and alkaline Phosphatases were found to be sensitive in phosphine treated and un-treated 4th instar larvae of Trogoderma granarium showing correlation of phosphine with induction/ inhibition of enzymes. It shows that they are involve in the mechanism of defense against fumigants by slowing down respiratory rate in resistant populations (Lhr, Hbd and Chi populations), which may be play important role in resistance development, while in susceptible populations (Khw, Rwp and Fqw populations) activity of the above enzymes decreases which indicate high respiratory rate in them. The data produced during this experiment can be used as indicator to understand the mechanism of toxicity in T. granarium.


Syed Shahid Ali
Supervisor

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