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Background: The newly developed herbal Formulation (LIV01-SSA-23), that has a combination of multiple medicinal herbs such as the powders of Solanum nigrum, Holarrhena antidysentrica, Tephrosia purpurea, Andrographis paniculata, Phyllanthus niruri, Tinospora cordifolia, Terminalia chebula, Asteracantha longifolia, Alstonia scholaris, Berberis aristata, Chichorium intybus, Picrorhiza kurroa, was tested for its antioxidant and free radical scavenging activity in vitro. This PHF has been traditionally used by the ayurvedic practioners in India, for the treatment of various liver complications and inflammatory disorders. Methodology: The developed formulation was tested for total reducing power and in vitro antioxidant activity by ABTS assay, Superoxide anion scavenging activity assay and Lipid per-oxidation assay. Conclusion: In conclusion, we strongly suggest that Polyherbal formulation are source of potential antioxidant for radical scavenging. The highly positive correlation of anti?radical scavenging activity and total polyphenolic content in Polyherbal compounds indicates that polyphenols are important components which could be used for the free radical scavenging activity. Further study is needed for isolation and characterization of the active moiety responsible for biological activity and to treat in various stress condition.