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Background: The streptococcus mutans and lactobacilli have been considered to be the most common causative bacteria for dental decay. Antibacterial action of polycarboxylate cement reinforced by different amount of fluoroapatite and calcium fluoride is important property for the filling restoration to prevent secondary caries and to evaluate these materials to be used as base filling in which the antibacterial activity is important property for successful filling and cementing material for crowns and bridges ,thus the aim of the study was the analysis the antimicrobial activity of polycarboxylate cement reinforced by different amount of fluoroapatite and calcium fluoride. Materials& methods: fluoroapatite materials and calcium fluoride were added to polycarboxylate cement at different ratios; 0% (Control), 5% ,10%,15%, 20%, 25% (by weight). Agar diffusion method will used to evaluate the antibacterial effect polycarboxylate cement and polycarboxylate cement reinforced by different amounts of fluoroapatite and calicium fluoirde (5% ,10%,15%, 20% and 25%) in this study two bacterial strains are used ; streptococcus mutans and lactobacilli which are cultured. A six holes (5mm depth and 4mm diameter) in each Petri dish( 20 petri dish for each of two micro organism of this study) ; 10 petri dish for polycarboxylate cement and polycarboxylate cement reinforced by different amounts of fluoroapatite (5% ,10%,15%, 20% and 25%) and 10 petri dish for polycarboxylate cement and polycarboxylate cement reinforced by different amounts of calicium fluoirde (5% ,10%,15%, 20% and 25% ) . in this study the material were added in these Wells (one Well for each ratio was used) and after incubation of these Petri dishes , the inhibition zones of each microorganism of this study were measured in millimeter around each of these Wells. Results: the conclusion n this study showed that the polycarboxylate cement and polycarboxylate cement reinforced by different amounts of fluoroapatite and calicium fluoride have strong antibacterial activity against streptococus mutans and lactobacilli Bacteria. Also the results showed that the increase the ratio of fluoroapatite will statistically increase their antibacterial activity aganist streptococcus mutans and lactobacilli as far as 20%.

In the northwestern part of the Zinder Region the study area the Continental Hamadien aquifer, tapped by numerous modern waterworks, serves as the primary source of drinking water for the local population. This is a densely populated area where water resource issues are particularly acute. Furthermore, the rural drinking water coverage rate in the region stands at 63.65%, compared to a national average of 56.6%. Groundwater quality in the area is characterized by high fluoride levels; this study was therefore undertaken with the primary objective of determining the percentage of boreholes with high fluoride concentrations and assessing their impact on local drinking water coverage. Chemical analysis of groundwater samples and subsequent data interpretation revealed that 23% of the sampled water points exceed the WHO limit for drinking water (1.5 mg/L), rendering the water unfit for human consumption. Furthermore, the fluoride found in the groundwater resources of the study area is of mineral origin and appears to stem from the fluorite (CaF2) content of the detrital reservoir, which is composed exclusively of weathering products from the crystalline massifs of the Nigerian Shield.